In-Source Collision-Induced Dissociation (CID) Improves Higher-Energy Collisional Dissociation (HCD)-Dependent Fragmentation of ADP-Ribosyl Peptides.

ABSTRACT:

Rationale

ADP-ribosylation is a posttranslational modification whose higher-energy collisional dissociation (HCD) products are dominated by complete or partial modification losses, complicating peptide sequencing and acceptor site localization efforts. We tested whether in-source collision-induced dissociation (CID) performed on a quadrupole-Orbitrap could convert ADPr to the smaller phosphoribose-H₂O derivative to facilitate HCD-dependent peptide sequencing.

Methods

ADP-ribosyl (ADPr) peptides derived from the human macrophage-like cell line THP-1 were analyzed on a quadrupole-Orbitrap. We monitored the dissociation of ADPr (+ 541.061 Da) to phosphoribosyl-H₂O (+ 193.997 Da) peptides while varying the source and high-field asymmetric waveform ion mobil

SUBMITTER: Kasai T 

PROVIDER: S-EPMC11617611 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature