Accurate drift-invariant single-molecule force calibration using the Hadamard variance.

ABSTRACT: Single-molecule force spectroscopy (SMFS) techniques play a pivotal role in unraveling the mechanics and conformational transitions of biological macromolecules under external forces. Among these techniques, multiplexed magnetic tweezers (MT) are particularly well suited to probe very small forces, ≤1 pN, critical for studying noncovalent interactions and regulatory conformational changes at the single-molecule level. However, to apply and measure such small forces, a reliable and accurate force-calibration procedure is crucial. Here, we introduce a new approach to calibrate MT based on thermal motion using the Hadamard variance (HV). To test our method, we perform bead-tether Brownian dynamics simulations that mimic our experimental system and compare the performance of the HV method agai

SUBMITTER: Pritzl SD 

PROVIDER: S-EPMC11617635 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature