Assay for Characterizing Adsorption-Properties of Surfaces (APS).

ABSTRACT: Analytes, from sample preparation, until entering an analytical instrument, are prone to adsorb to surfaces, driven by the chemical properties of the surface and the liquids they are dissolved in. This problem can be addressed with internal standards when a single or few known analytes are quantified that are usually not available in omics. However, minimal to no loss of analytes is the aim. Here, we present a novel assay for qualifying and quantifying interactions responsible for adsorption of molecules to surfaces (APS) by using LC-MS/MS-based differential quantitative analysis. To reflect a broad range of chemical interactions with surfaces, a reference mixture of thousands of tryptic peptides, with known compositions was selected, representing a variety of different chemical characteri

SUBMITTER: Siebels B 

PROVIDER: S-EPMC11618038 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature