Extended Spherical Diffusion Theory: Electrochemiluminescence Imaging Analysis of Diffusive Molecules from Spherical Biosamples.

ABSTRACT: Spherical biosamples such as immunobeads, cells, and cell aggregates have been widely used in bioapplications. The bioactivity of individual spherical biosamples in highly sensitive assays and individual analyses must be evaluated in a high-throughput manner. Electrochemiluminescence (ECL) imaging was recently proposed for the high-throughput analysis of diffusive molecules from spherical biosamples. ECL imaging involves the placing of spherical biosamples on a flat electrode filled with a solution. The biosamples produce (or consume) biological/chemical molecules such as H₂O₂ and O₂, which diffuse to form a concentration gradient at the electrode. The ECL signals from the molecules are then measured to obtain the concentration profile, which allows the flu

SUBMITTER: Ino K 

PROVIDER: S-EPMC11618750 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature