Spatially-Resolved Organoid Transfection by Porous Silicon-Mediated Optoporation.

ABSTRACT: Engineering the spatial organisation of organotypic cultures is pivotal for refining tissue models that are useful for gaining deeper insights into complex, non-cell autonomous processes. These advanced models are key to improving the understanding of fundamental biological mechanisms and therapeutic strategies. Controlling gene regulation through spatially-resolved delivery of nucleic acids provides an attractive approach to produce such tissue models. An emerging strategy for spatially-resolved transfection uses photosensitizing nanoparticles coupled with laser pulses to optoporate cells in culture and locally mediate gene delivery. However, localized optoporation in 3D systems remains challenging. Here we propose a solution to this longstanding hurdle, demonstrating that porous silicon

SUBMITTER: Spiteri C 

PROVIDER: S-EPMC11619231 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature