Pulsed radiofrequency alleviates neuropathic pain by upregulating MG53 to inhibit microglial activation.

ABSTRACT:

Background

Patients with neuropathic pain (NP) have significantly lower quality of life. Because the pathophysiology of NP is not fully understood, there is a lack of effective treatment for it in clinic. This study set out to investigate the precise mechanism by which pulsed radiofrequency (PRF) alleviated NP.

Method

The rat models of chronic constriction injury of the sciatic nerve (CCI) were established to simulate the occurrence of NP, following with measuring MWT and TWL to evaluate the pain of the rats. HE staining was utilized to observe the rat spinal cord tissue pathology. The expression of MG53, ATF4 and CHOP was evaluated by qRT-PCR and WB, while the expression of inflammatory factors was measured by ELISA. In addition, immunofluorescence assay was used to detect

SUBMITTER: Huang Y 

PROVIDER: S-EPMC11619262 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature