Evaluation of a microfluidic-based point-of-care prototype with customized chip for detection of bacterial clusters.

ABSTRACT: Bacterial infection clusters cause high mortality rates and healthcare costs due to excessive therapy and hygiene measures. The aim of this study was to develop an automated real-time PCR-based point-of-care (POC) system with a customized microfluidic chip that facilitates fast detection of bacterial cluster isolates by targeting cluster-specific single-nucleotide polymorphisms (SNPs). For cluster detection of Acinetobacter baumannii, Staphylococcus aureus, and Escherichia coli, nine TaqMan real-time PCR assays targeting cluster-specific SNPs were designed. Additionally, for DNA input control, a universal PCR amplifying the 16S rDNA was constructed. All reactions were implemented into a microfluidic chip that was analyzed by a POC prototype enabling automated sample pr

SUBMITTER: Treffon J 

PROVIDER: S-EPMC11619353 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature