New PALM-compatible integration vectors for use in the Gram-positive model bacterium <i>Bacillus subtilis</i>.

ABSTRACT: Improvements in super-resolution and single-molecule techniques, along with the development of new fluorescent proteins and labeling methods, have allowed super-resolution imaging of bacterial cells. Cloning vectors are important tools for engineering fluorescent fusions and perform efficient labeling. Here, we report the construction of four photoactivated localization microscopy (PALM)-compatible integration plasmids for the Gram-positive model organism Bacillus subtilis. These plasmids carry genes encoding either the photoswitchable green fluorescent protein dronPA or the photoactivatable red fluorescent protein PAmCherry1, codon-optimized or not for expression in B. subtilis. For fast and flexible cloning, multiple cloning sites were added at both the C-terminal and the N

SUBMITTER: Altinoglu I 

PROVIDER: S-EPMC11619463 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature