Biofilm matrix regulation by <i>Candida glabrata</i> Zap1 under acidic conditions: transcriptomic and proteomic analyses.

ABSTRACT: The vaginal acidic environment potentiates the formation of Candida glabrata biofilms, leading to complicated and recurrent infections. Importantly, the production of matrix is known to contribute to the recalcitrant features of Candida biofilms. In this study, we reveal that Zap1 regulates the matrix of C. glabrata acidic biofilms and analyzed the modulation of their transcriptome (by microarrays) and matrix proteome (by LC-MS/MS) by Zap1. For that, the deletion mutant zap1Δ and its complemented strain zap1Δ::ZAP1 were constructed, and their biofilms were developed at pH 4 (adjusted with lactic acid). The results revealed that Zap1 is a negative regulator of the total amount of protein and carbohydrate in the biofilm matrix. Accordingly, various g

SUBMITTER: Goncalves B 

PROVIDER: S-EPMC11619577 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature