Bmlark is essential for embryonic development.

ABSTRACT:

Background

Transcription factor lark has been demonstrated to play multiple functions in Drosophila, but the function of this gene in embryonic development remains to be elucidated.

Results

In this study, the CRISPR/Cas9 gene-editing method was used to construct a Bmlark mutant strain of Bombyx mori to investigate the roles of this gene. The results showed that the homozygous mutant Bmlark^-/- was lethal. The Bmlark^-/- embryos showed obvious developmental defects, such as defective sclerotization and melanization of the exoskeleton. A transcriptomic comparison of Bmlark^-/- and wild-type embryos showed that the differentially expressed genes were mainly enriched in the structure and metabolic processes of chitin and cuticles. While the expressi

SUBMITTER: Peng Y 

PROVIDER: S-EPMC11619667 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature