Analysis of early childhood intestinal microbial dynamics in a continuous-flow bioreactor.

ABSTRACT:

Background

The human gut microbiota is inoculated at birth and undergoes a process of assembly and diversification during the first few years of life. Studies in mice and humans have revealed associations between the early-life gut microbiome and future susceptibility to immune and metabolic diseases. To resolve microbe and host contributing factors to early-life development and to disease states requires experimental platforms that support reproducible, longitudinal, and high-content analyses.

Results

Here, we deployed a continuous single-stage chemostat culture model of the human distal gut to study gut microbiota from 18- to 24-month-old children integrating both culture-dependent and -independent methods. Chemostat cultures recapitulated multiple aspects of the fecal mic

SUBMITTER: Granato A 

PROVIDER: S-EPMC11619690 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature