Precise genome editing underlines the distinct contributions of mutations in <i>ERG11</i>, <i>ERG3</i>, <i>MRR1</i>, and <i>TAC1</i> genes to antifungal resistance in <i>Candida parapsilosis</i>.

ABSTRACT: Candida parapsilosis has recently emerged as a major threat due to the worldwide emergence of fluconazole-resistant strains causing clonal outbreaks in hospitals and poses a therapeutic challenge due to the limited antifungal armamentarium. Here, we used precise genome editing using CRISPR-Cas9 to gain further insights into the contribution of mutations in ERG11, ERG3, MRR1, and TAC1 genes and the influence of allelic dosage to antifungal resistance in C. parapsilosis. Seven of the most common amino acid substitutions previously reported in fluconazole-resistant clinical isolates (including Y132F in ERG11) were engineered in two fluconazole-susceptible C. parapsilosis lineages (ATCC 22019 and STZ5). Each mutant was then challenged

SUBMITTER: Hartuis S 

PROVIDER: S-EPMC11620491 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature