Measuring the rate of NADPH consumption by glutathione reductase in the cytosol and mitochondria.

ABSTRACT:

Background

NADPH is an essential co-factor supporting the function of enzymes that participate in both inflammatory and anti-inflammatory pathways in myeloid cells, particularly macrophages. Although individual NADPH-dependent pathways are well characterized, how these opposing pathways are co-regulated to orchestrate an optimized inflammatory response is not well understood. To investigate this, techniques to track the consumption of NADPH need to be applied. Deuterium tracing of NADPH remains the gold standard in the field, yet this setup of mass-spectrometry is technically challenging and not readily available to most research groups. Furthermore, NADPH pools are compartmentalized in various organelles with no known membrane transporters, suggesting that NADPH-dependent pathways

SUBMITTER: Ting KKY 

PROVIDER: S-EPMC11620681 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature