The Smc5/6 complex counteracts R-loop formation at highly transcribed genes in cooperation with RNase H2.

ABSTRACT: The R-loop is a common transcriptional by-product that consists of an RNA-DNA duplex joined to a displaced strand of genomic DNA. While the effects of R-loops on health and disease are well established, there is still an incomplete understanding of the cellular processes responsible for their removal from eukaryotic genomes. Here, we show that a core regulator of chromosome architecture -the Smc5/6 complex- plays a crucial role in the removal of R-loop structures formed during gene transcription. Consistent with this, budding yeast mutants defective in the Smc5/6 complex and enzymes involved in R-loop resolution show strong synthetic interactions and accumulate high levels of RNA-DNA hybrid structures in their chromosomes. Importantly, we demonstrate that the Smc5/6 complex acts on specifi

SUBMITTER: Roy S 

PROVIDER: S-EPMC11620742 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature