Distinct T-cell receptor (TCR) gene segment usage and MHC-restriction between foetal and adult thymus.

ABSTRACT: Here, we sequenced rearranged TCRβ and TCRα chain sequences in CD4⁺CD8⁺ double positive (DP), CD4⁺CD8^- single positive (SP4) and CD4^-CD8⁺ (SP8) thymocyte populations from the foetus and young adult mouse. We found that life-stage had a greater impact on TCRβ and TCRα gene segment usage than cell-type. Foetal repertoires showed bias towards 3'TRAV and 5'TRAJ rearrangements in all populations, whereas adult repertoires used more 5'TRAV gene segments, suggesting that progressive TCRα rearrangements occur less frequently in foetal DP cells. When we synchronised young adult DP thymocyte differentiation by hydrocortisone treatment the new recovering DP thymocyte population showed more foetal-like 3'TRAV and 5'TRAJ gene segment usa

SUBMITTER: Rowell J 

PROVIDER: S-EPMC11620746 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature