<i>Gluconacetobacter diazotrophicus</i> AZ0019 requires functional <i>nifD</i> gene for optimal plant growth promotion in tomato plants.

ABSTRACT: Gluconacetobacter diazotrophicus is a nitrogen fixing bacterium able to colonise a wide range of host plants and is marketed as a biofertiliser due to its ability to promote plant growth. This study aims to investigate how biological nitrogen fixation (BNF) competency affects the growth promotion of inoculated tomato plants and to describe the colonisation mechanism of this bacterium in dicot systems. A nitrogen fixation impaired mutant (Gd nifD^- ) was produced by disrupting the nifD gene, which encodes the nitrogenase Mo-Fe subunit, in order to assess its plant growth promotion (PGP) capability in comparison to G. diazotrophicus wild type strain (Gd WT). Furthermore, tagged strains were employed to monitor the colonisation process through qPCR analys

SUBMITTER: Pallucchini M 

PROVIDER: S-EPMC11620874 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature