Image processing tools for petabyte-scale light sheet microscopy data.

ABSTRACT: Light sheet microscopy is a powerful technique for high-speed three-dimensional imaging of subcellular dynamics and large biological specimens. However, it often generates datasets ranging from hundreds of gigabytes to petabytes in size for a single experiment. Conventional computational tools process such images far slower than the time to acquire them and often fail outright due to memory limitations. To address these challenges, we present PetaKit5D, a scalable software solution for efficient petabyte-scale light sheet image processing. This software incorporates a suite of commonly used processing tools that are optimized for memory and performance. Notable advancements include rapid image readers and writers, fast and memory-efficient geometric transformations, high-performance Richar

SUBMITTER: Ruan X 

PROVIDER: S-EPMC11621031 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature