Natural infection of hybrid sturgeon (Acipenser baerii♀×Acipenser schrenckii♂) with Nocardia seriolae and white sturgeon iridovirus: pathological and transcriptomic analyses.

ABSTRACT:

Introduction

In August 2023, hybrid sturgeons (Acipenser baerii♀×Acipenser schrenckii♂) cultured in Sichuan, China, showed infectious disease symptoms, including ulcers, liver and spleen nodules, and high mortality rates.

Methods

Pathogenic bacteria were isolated from the liver of diseased sturgeons and analyzed for their phenotypic and molecular traits. Furthermore, iridovirus-specific TaqMan real-time PCR (RT-PCR) analyses were conducted. The histopathological characteristics were analyzed using paraffin sectioning and transmission electron microscopy (TEM). Transcriptome sequencing was performed to elucidate the impact of pathogen exposure and immune response profiles in infected sturgeon.

Results

Pathogenic bacteria isolation and phylogenetic analyses of th

SUBMITTER: Ni L 

PROVIDER: S-EPMC11621106 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature