Single Sperm RNA signatures reveal MicroRNA biomarkers for male subfertility.

ABSTRACT:

Purpose

To investigate small RNA profiles in sperm, identify stable miRNA patterns unique to sperm, and assess the behavior of consistently expressed miRNAs in sperm from subfertile men compared to fertile controls.

Methods

The small RNA profiles of single sperm from four proven fertile men were analyzed using Small RNA next-generation sequencing (NGS). Subsequently, a specific set of miRNAs was validated using RT-qPCR on additional sperm samples from 65 subfertile men from an infertility clinic and 30 proven fertile men.

Results

Small RNA sequencing revealed a diverse range of sperm small RNA biotypes, including miRNAs. The mapped read percentage ranged from 22.19% for single sperm to 83.29% for enriched sperm samples used at different RNA concentrations. In single s

SUBMITTER: Abu-Halima M 

PROVIDER: S-EPMC11621271 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature