Label-free single-cell RNA multiplexing leveraging genetic variability.

ABSTRACT: Single cell RNA sequencing has provided unprecedented insights into the molecular cues and cellular heterogeneity underlying human disease. However, the high costs and complexity of single cell methods remain a major obstacle for generating large-scale human cohorts. Here, we compare current state-of-the-art single cell multiplexing technologies, and provide a widely applicable demultiplexing method, SoupLadle, that enables simple, yet robust high-throughput multiplexing leveraging genetic variability of patients.

SUBMITTER: Hoeft K 

PROVIDER: S-EPMC11621319 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature