Quantifying acyl-chain diversity in isobaric compound lipids containing monomethyl branched-chain fatty acids.

ABSTRACT: Compound lipids comprise a diverse group of metabolites present in living systems, and metabolic- and environmentally-driven structural distinctions across this family are increasingly linked to biological function. However, methods for deconvoluting these often isobaric lipid species are lacking or require specialized instrumentation. Notably, acyl-chain diversity within cells may be influenced by nutritional states, metabolic dysregulation, or genetic alterations. Therefore, a reliable, validated method of quantifying structurally similar even-, odd-, and branched-chain acyl groups within intact compound lipids will be invaluable for gaining molecular insights into their biological functions. Here we demonstrate the chromatographic resolution of isobaric lipids containing distinct combin

SUBMITTER: Green CR 

PROVIDER: S-EPMC11621494 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature