Identification of a dengue 2 virus envelope protein receptor in <i>Aedes aegypti</i> critical for viral midgut infection.

ABSTRACT: The establishment of a productive dengue virus (DENV) infection in the midgut epithelial cells of Aedes aegypti is critical for the viral transmission cycle. The hypothesis that DENV virions interact directly with specific mosquito midgut proteins was explored. We found that DENV serotype 2 (DENV2) pretreated with trypsin interacted with a single 31 kDa protein, identified as AAEL011180 by protein mass spectrometry. This putative receptor is a highly conserved protein and has orthologs in culicine and anopheline mosquitoes. We confirmed that impairing the expression of AAEL011180 in the midgut of Ae. aegypti females abolished the interaction with DENV2, and the virus also bound to immobilized recombinant purified receptor. Furthermore, recombinant DENV2 surface E glycoprotein

SUBMITTER: Kantor AM 

PROVIDER: S-EPMC11621822 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature