Genes involved in osteogenic differentiation induced by low‑intensity pulsed ultrasound in goldfish scales.

ABSTRACT: The teleost scale is a unique calcified tissue that contains osteoclasts, osteoblasts, osteocytes and the bone matrix, similar to mammalian bone. Here, the effects of low-intensity pulsed ultrasound (LIPUS) on osteoblasts and osteoclasts in goldfish scales were investigated. Scales were treated with LIPUS, which is equivalent to use under clinical conditions (30 mW/cm² for 20 min), then cultured at 15˚C. Alkaline phosphatase activity, a marker of osteoblasts, or tartrate-resistant acid phosphatase (TRAP) activity, a marker of osteoclasts was measured. The gene expression profile was examined using RNA-sequencing. Gene network and biological function analyses were performed using the Ingenuity^® Pathways Knowledge Base. A single exposure of LIPUS significantly increased

SUBMITTER: Tabuchi Y 

PROVIDER: S-EPMC11621920 | biostudies-literature | 2025 Feb

REPOSITORIES: biostudies-literature