Investigating the photophysical properties of rhodamines using a spectroscopic single-molecule fluorescence method.

ABSTRACT: The photophysical properties of rhodamine molecules play a critical role in their performance across various applications. The spectroscopic single-molecule fluorescence (sSMF) technique overcomes the limitations of conventional SMF by distinguishing individual fluorophores based on their emission spectra. This enables precise measurement and direct comparison of photophysical properties among distinct molecules under identical conditions, without requiring separation of molecules. In this study, using a custom sSMF instrument, we successfully identified individual rhodamine B molecules and their various N-dealkylated intermediates, allowing for simultaneous investigation of their photophysical properties. Notably, we observed that rhodamine B undergoing a single dealkylation step e

SUBMITTER: Deng S 

PROVIDER: S-EPMC11622037 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature