A Streamlined Workflow for Microscopy-Driven MALDI Imaging Mass Spectrometry Data Collection.

ABSTRACT: Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a rapidly advancing technology for biomedical research. As spatial resolution increases, however, so do acquisition time, file size, and experimental cost, which increases the need to perform precise sampling of targeted tissue regions to optimize the biological information gleaned from an experiment and minimize wasted resources. The ability to define instrument measurement regions based on key tissue features and automatically sample these specific regions of interest (ROIs) addresses this challenge. Herein, we demonstrate a workflow using standard software that allows for direct sampling of microscopy-defined regions by MALDI IMS. Three case studies are included, highlighting different methods for defin

SUBMITTER: Esselman AB 

PROVIDER: S-EPMC11622233 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature