Charging of DNA Complexes in Positive-Mode Native Electrospray Ionization Mass Spectrometry.

ABSTRACT: Native mass spectrometry (nMS) provides insights into the structures and dynamics of biomacromolecules in their native-like states by preserving noncovalent interactions through "soft" electrospray ionization (ESI). For native proteins, the number of charges that are acquired scales with the surface area and mass. Here, we explore the effect of highly negatively charged DNA on the ESI charge of protein complexes and find a reduction of the mass-to-charge ratio as well as a greater variation. The charge state distributions of pure DNA assemblies show a lower mass-to-charge ratio than proteins due to their greater density in the gas phase, whereas the charge of protein-DNA complexes can additionally be influenced by the distribution of the ESI charges, ion pairing events, and collapse of the

SUBMITTER: Abramsson ML 

PROVIDER: S-EPMC11622369 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature