Enzymatic Synthesis of Isotopically Labeled Hydrogen Peroxide for Mass Spectrometry-Based Applications.

ABSTRACT: Methionine oxidation is involved in multiple biological processes including protein misfolding and enzyme regulation. However, it is often challenging to measure levels of methionine oxidation by mass spectrometry, in part due to the prevalence of artifactual oxidation that occurs during the sample preparation and ionization steps of typical proteomic workflows. Isotopically labeled hydrogen peroxide (H₂¹⁸O₂) can be used to block unoxidized methionines and enables accurate measurement of in vivo levels of methionine oxidation. However, H₂¹⁸O₂ is an expensive reagent that can be difficult to obtain from commercial sources. Here, we report a method for synthesizing H₂¹⁸O₂ in-house. Gluco

SUBMITTER: Hoare M 

PROVIDER: S-EPMC11622373 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature