Fibro-adipogenic progenitor cells in skeletal muscle unloading: metabolic and functional impairments.

ABSTRACT:

Background

Skeletal muscle resident fibro-adipogenic progenitor cells (FAPs) control skeletal muscle regeneration providing a supportive role for muscle stem cells. Altered FAPs characteristics have been shown for a number of pathological conditions, but the influence of temporary functional unloading of healthy skeletal muscle on FAPs remains poorly studied. This work is aimed to investigate how skeletal muscle disuse affects the functionality and metabolism of FAPs.

Methods

Hindlimb suspension (HS) rat model employed to investigate muscle response to decreased usage. FAPs were purified from m. soleus functioning muscle (Contr) and after functional unloading for 7 and 14 days (HS7 and HS14). FAPs were expanded in vitro, and tested for: immunophenotype; in vitro expansion ra

SUBMITTER: Sorokina M 

PROVIDER: S-EPMC11622572 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature