The miR-6240 target gene Igf2bp3 promotes myoblast fusion by enhancing myomaker mRNA stability.

ABSTRACT:

Background

Myoblast fusion plays a crucial role in myogenesis. Insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) functions as an RNA N⁶-methyladenosine reader and exerts important roles in various biological processes. While our prior study suggested Igf2bp3 contributes to myogenesis, its molecular regulatory mechanism is largely unclear.

Methods

Real-time quantitative polymerase chain reaction (RT-qPCR) and western blot were used for gene expression analysis. siRNA and CRISPRi technologies were conducted to knockdown the expression of Igf2bp3. CRISPR/Cas9 technology was performed to knockout Igf2bp3. The Igf2bp3 overexpression vector was designed using the pcDNA3.1(+) vector. Immunofluorescence detection was employed for subcellular localization and c

SUBMITTER: Huang Y 

PROVIDER: S-EPMC11622686 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature