CXXC5 stabilizes DNA methylation patterns in mouse embryonic stem cells.

ABSTRACT:

Aims

Mammalian genomes encode 12 proteins that contain a CXXC zinc finger domain. Most members of this family are large multi-domain proteins that function in the control of DNA methylation and histone methylation patterns. CXXC5 is a smaller member of the family, along with its closest homologue CXXC4. These two proteins lack known catalytic domains. Here, we have characterized CXXC5 in mouse embryonic stem (ES) cells.

Materials & methods

We used gene knockouts, RNA sequencing, and DNA methylation analysis by whole-genome bisulfite sequencing.

Results & conclusions

We show that CXXC5 is a nuclear protein that interacts with 5-methylcytosine oxidases (TET proteins). Removal of CXXC5 from ES cells leads to very few changes in gene expression. CXXC5 extensively colocali

SUBMITTER: Jin SG 

PROVIDER: S-EPMC11622772 | biostudies-literature | 2024 Nov-Nov

REPOSITORIES: biostudies-literature