High-fidelity and iterative affinity extraction of hyaluronan.

ABSTRACT: The glycosaminoglycan hyaluronan (HA) serves a variety of crucial physiological functions in vertebrates. Synthesized at the plasma membrane and secreted into the extracellular environment, HA polymers span a wide range of molecular weights (MW) that define their activity through a notable size-function relationship. Analytical technologies for determining HA MW distributions typically require selective extraction from complex biofluids or tissues. A common method for achieving this is immunoprecipitation-like pull-down using specific HA-binding proteins bound to magnetic beads. Here, we present a systematic investigation of experimental variables involved in this process, leading to an affinity extraction protocol that enables iterative bead reuse and reagent lifetime maximization, thereb

SUBMITTER: Erxleben DA 

PROVIDER: S-EPMC11623434 | biostudies-literature | 2024 Oct-Dec

REPOSITORIES: biostudies-literature