CRISPRi Screen Uncovers lncRNA Regulators of Human Monocyte Growth.

ABSTRACT: Long noncoding RNAs are emerging as critical regulators of biological processes. While there are over 20,000 lncRNAs annotated in the human genome we do not know the function for the majority. Here we performed a high-throughput CRISPRi screen to identify those lncRNAs that are important in viability in human monocytes using the cell line THP1. We identified a total of 38 hits from the screen and validated and characterized two of the top intergenic hits. The first is a lncRNA neighboring the macrophage viability transcription factor IRF8 (RP11-542M13.2 hereafter referred to as long noncoding RNA regulator of monocyte proliferation, LNCRMP) and the second is a lncRNA called OLMALINC (oligodendrocyte maturation-associated long intervening non-coding RNA) that was previo

SUBMITTER: Flores-Arena C 

PROVIDER: S-EPMC11623541 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature