Detection of human IgG antibodies against <i>Mycoplasma genitalium</i> using a recombinant MG075 antigen.

ABSTRACT: Mycoplasma genitalium is a sexually transmitted pathogen, which can cause a range of reproductive tract diseases in both men and women. To disentangle the relationship between M. genitalium infection(s) and subsequent reproductive health complications at the population level, accurate serological tools are needed. The major challenge in developing specific M. genitalium serological tests is the extensive cross-reactivity with the closely related ubiquitous respiratory tract pathogen, M. pneumoniae. In this report, we describe the development of an immunoblot assay based on a recombinant fragment of the M. genitalium MG075 protein present in lipid-associated membrane extracts. A sensitivity of 87.1% was achieved based on testing antibody responses in sera

SUBMITTER: Kildemoes AO 

PROVIDER: S-EPMC11623746 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature