Endothelial Chromatin-Remodeling Enzymes Regulate the Production of Critical ECM Components During Murine Lung Development.

ABSTRACT:

Background

The chromatin-remodeling enzymes BRG1 (brahma-related gene 1) and CHD4 (chromodomain helicase DNA-binding protein 4) independently regulate the transcription of genes critical for vascular development, but their coordinated impact on vessels in late-stage embryos has not been explored.

Methods

In this study, we genetically deleted endothelial Brg1 and Chd4 in mixed background mice (Brg1^fl/fl;Chd4^fl/fl;VE-Cadherin-Cre), and littermates that were negative for Cre recombinase were used as controls. Tissues were analyzed by immunostaining, immunoblot, and flow cytometry. Quantitative reverse transcription polymerase chain reaction was used to determine gene expression, and chromatin immunoprecipita

SUBMITTER: Wu ML 

PROVIDER: S-EPMC11624602 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature