Native mass spectrometry prescreening of G protein-coupled receptor complexes for cryo-EM structure determination.

ABSTRACT: G protein-coupled receptors (GPCRs) are essential transmembrane proteins playing key roles in human health and disease. Understanding their atomic-level molecular structure and conformational states is imperative for advancing drug development. Recent breakthroughs in single-particle cryogenic electron microscopy (cryo-EM) have propelled the structural biology of GPCRs into a new era. Nevertheless, the preparation of suitable GPCR samples and their complexes for cryo-EM analysis remains challenging due to their poor stability and highly dynamic nature. Here, we present our online buffer exchange-native MS method combined with Direct Mass Technology (OBE-nMS+DMT) which facilitates high-throughput analysis and guides sample preparation. We applied this method to optimize the GPR119-G_s</

SUBMITTER: Kim D 

PROVIDER: S-EPMC11625002 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature