Tissue-specific inducible IL-33 expression elicits features of eosinophilic esophagitis.

ABSTRACT:

Background

IL-33 is a type 2 inflammatory cytokine that is elevated in the esophageal epithelium of eosinophilic esophagitis (EoE) subjects. We previously developed a mouse model of EoE dependent on constitutive overexpression of IL-33 from the esophageal epithelium (EoE33).

Objective

Our objective was to develop an inducible, IL-33-dependent model of EoE and examine induction of EoE-associated pathology.

Methods

We utilized a tetracycline-inducible system to express IL-33 in the esophagus by generating 2 transgenic mice. The first (iSophagus) expresses a reverse tetracycline transactivator from the esophageal epithelium. The second (TRE33) features a tetracycline response element driving expression of IL-33. When crossed, these mice generate an inducible model of EoE

SUBMITTER: Pyon GC 

PROVIDER: S-EPMC11625005 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature