Quantitative analysis of heme and hemoglobin for the detection of intravascular hemolysis.

ABSTRACT:

Background

Intravascular hemolysis is associated with massive release of hemoglobin and consequently labile heme into the blood, resulting in prothrombotic and proinflammatory events in patients. Though heme is well-known to participate in these adverse effects, it is not monitored. Instead, haptoglobin and hemoglobin serve as clinical biomarkers. The quantification of labile heme together with hemoglobin, however, should be considered in clinical diagnosis as well, to obtain a complete picture of the hemolytic state in patients. So far, quantification techniques for labile heme were not yet systematically analyzed and compared for their clinical application potential, especially in the presence of hemoglobin.

Results

Two commercial assays (Heme Assay Kit®, Hemin Assay Kit®)

SUBMITTER: Hopp MT 

PROVIDER: S-EPMC11625178 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature