Incretin-responsive human pancreatic adipose tissue organoids: A functional model for fatty pancreas research.

ABSTRACT:

Objective

Infiltration of adipocytes into the pancreatic parenchyma has been linked to impaired insulin secretion in individuals with increased genetic risk of T2D and prediabetic conditions. However, the study of this ectopic fat depot has been limited by the lack of suitable in vitro models.

Methods

Here, we developed a novel 3D model of functionally mature human pancreatic adipose tissue organoids by aggregating human pancreatic adipose tissue-derived stromal vascular fraction (SVF) cells into organoids and differentiating them over 19 days.

Results

These organoids carry biological properties of the in situ pancreatic fat, presenting levels of adipogenic markers comparable to native pancreatic adipocytes and improved lipolytic and anti-lipolytic response compared t

SUBMITTER: Lorza-Gil E 

PROVIDER: S-EPMC11625218 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature