Protocol for isolating and culturing neonatal murine cardiomyocytes.

ABSTRACT: The isolation and culture of neonatal murine cardiac cells are valuable techniques for studying their properties and molecular mechanisms in response to various treatments or conditions. Here, we present a protocol for isolating a high yield of viable neonatal murine cardiac cells, including functional, beating cardiomyocytes. We describe the steps of heart extraction, washing and pre-digestion, digestion, and cell seeding. We detail procedures for mechanical and enzymatic digestions, conducted in a controlled environment within a cell culture incubator. For complete details on the use and execution of this protocol, please refer to Bongiovanni et al.¹.

SUBMITTER: Bongiovanni C 

PROVIDER: S-EPMC11625229 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature