Associations between glycan signature alterations and the cellular antigenic properties of passaged chondrocytes.

ABSTRACT:

Background

Cartilage repair is a significant clinical challenge because of the limited intrinsic healing capacity. Current therapeutic strategies, such as cell transplantation therapy, aim to overcome this challenge by replacing damaged tissue with healthy cells. However, the long-term survival and functionality of transplanted cells remain major hurdles.

Objective

This study investigated the impact of chondrocyte passaging on glycan profiles and their antigenic properties. We hypothesized that alterations in glycan composition due to passaging may contribute to the enhanced ability to activate macrophages, thereby affecting the outcome of cell transplantation therapy.

Methods

Peritoneal macrophages and primary articular chondrocytes were isolated from C57BL/6 mice to

SUBMITTER: Homan K 

PROVIDER: S-EPMC11625746 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature