Dorsal root ganglia CSF1⁺ neuronal subtypes have different impact on macrophages and microglia after spared nerve injury.

ABSTRACT:

Background and aims

Colony-stimulating factor 1 (CSF1) is a growth factor secreted by dorsal root ganglia (DRG) neurons important for DRG macrophages and spinal cord (SC) microglia injury-induced proliferation and activation, specifically released after spared nerve injury (SNI). In this study, we investigated if SNI-induced CSF1 expression and perineuronal rings of macrophages around mouse DRG neurons vary between L3-L5 DRG and with the neuronal type, and if the CSF1⁺ neuronal projections at the SC dorsal horns were associated with an increased microglial number in the corresponding laminae.

Methods

Seven days after surgery, L3-L5 DRG as well as their corresponding segments at the SC level were collected, frozen, and cut. DRG sections were double-immunostained us

SUBMITTER: Grosu AV 

PROVIDER: S-EPMC11625985 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature