Oxygen matters: Unraveling the role of oxygen in the neuronal response to cisplatin.

ABSTRACT:

Background and aims

Cell culture is a fundamental experimental tool for understanding cell physiology. However, translating these findings to in vivo settings has proven challenging. Replicating donor tissue conditions, including oxygen levels, is crucial for achieving meaningful results. Nevertheless, oxygen culture conditions are often overlooked, particularly in the context of chemotherapy-induced neurotoxicity.

Methods

In this study, we investigated the role of oxygen levels in primary neuronal cultures by comparing neuronal performance under cisplatin exposure (1 μg/mL) in supraphysiological normoxia (representing atmospheric conditions in a standard incubator; 18.5% O₂) and physioxia (representing physiologic oxygen conditions in nervous tissue; 5% O_2</

SUBMITTER: Crugeiras J 

PROVIDER: S-EPMC11625991 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature