Cyclosporine and fedratinib combination therapy via modulating Th17/Treg balance in Rat model of membranous glomerulonephritis.

ABSTRACT: A progressive kidney disease associated with inflammation and the immune system is called membrane glomerulonephritis (MGN). The present study investigatedthe combination of cyclosporine and fedratinib on Th17/regulatory T cells (Tregs) in rat models of MGN. Rats were given several doses of anti-Fx1A to induce MGN, and the resultant five groups of rats were fedratinib-cyclosporin receiving PHN rats, fedratinib, cyclosporin, and healthy rats. Following that, the blood's biochemistry was ascertained, and splenocytes were separated to use flow cytometry to look into the proportion of Th17 and Treg cells in the blood. A real-time PCR test was used to assess the corresponding Tregs and Th17 cell transcription factors andtheir related cytokine gene expressions. Finally, serum analysis was employ

SUBMITTER: Ghassabi A 

PROVIDER: S-EPMC11626047 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature