Electrochemical cofactor recycling of bacterial microcompartments.

ABSTRACT: Bacterial microcompartments (BMCs) are prokaryotic organelles that consist of a protein shell which sequesters metabolic reactions in its interior. While most of the substrates and products are relatively small and can permeate the shell, many of the encapsulated enzymes require cofactors that must be regenerated inside. We have analyzed the occurrence of an enzyme previously assigned as a cobalamin (vitamin B₁₂) reductase and, curiously, found it in many unrelated BMC types that do not employ B₁₂ cofactors. We propose Nicotinamide adenine dinucleotide (NAD+) regeneration as the function of this enzyme and name it Metabolosome Nicotinamide Adenine Dinucleotide Hydrogen (NADH) dehydrogenase (MNdh). Its partner shell protein BMC-T^SE (tandem domain BMC shell p

SUBMITTER: Sutter M 

PROVIDER: S-EPMC11626177 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature