Proteome-wide bioinformatic annotation and functional validation of the monotopic phosphoglycosyl transferase superfamily.

ABSTRACT: Phosphoglycosyl transferases (PGTs) are membrane proteins that initiate glycoconjugate biosynthesis by transferring a phospho-sugar moiety from a soluble nucleoside diphosphate sugar to a membrane-embedded polyprenol phosphate acceptor. The centrality of PGTs in complex glycan assembly and the current lack of functional information make these enzymes high-value targets for biochemical investigation. In particular, the small monotopic PGT family is exclusively bacterial and represents the minimal functional unit of the monotopic PGT superfamily. Here, we combine a sequence similarity network analysis with a generalizable, luminescence-based activity assay to probe the substrate specificity of this family of monoPGTs in the bacterial cell-membrane fraction. This strategy allows us to identif

SUBMITTER: Durand T 

PROVIDER: S-EPMC11626204 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature