Discovery of thiazostatin D/E using UPLC-HR-MS2-based metabolomics and σ-factor engineering of Actinoplanes sp. SE50/110.

ABSTRACT: As the natural producer of acarbose, Actinoplanes sp. SE50/110 has high industrial relevance. Like most Actinobacteria, the strain carries several more putative biosynthetic gene clusters (BGCs) to produce further natural products, which are to be discovered. Applying a metabolomics-guided approach, we tentatively identified five further compounds that are produced by the strain: watasemycin, thiazostatin, isopyochelin, pulicatin, and aerugine. A comparison of the genomic context allowed the identification of the putative BGC, which is highly similar to the watasemycin biosynthetic gene cluster of Streptomyces venezuelae. In addition to the identified molecules, a thiazostatin-like compound was found. Isolation and structure elucidation with 1D and 2D NMR and HRMS were applie

SUBMITTER: Schluter L 

PROVIDER: S-EPMC11626248 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature