Revised method for preparation of simulated body fluid for assessment of the apatite-forming ability of bioactive materials: proposal of mixing two stock solutions.

ABSTRACT: Bioactive glasses and glass-ceramics exhibit osteoconductivity, which is the ability to form a direct bond with living bone tissue. This property is typically assessed by observing the formation of a hydroxyapatite layer in vitro using simulated body fluid (SBF), a solution designed to mimic the inorganic constituents of human blood plasma. SBF was developed by Kokubo et al. (T. Kokubo, H. Kushitani, S. Sakka, T. Kitsugi and T. Yamamuro, J. Biomed. Mater. Res., 1990, 24, 721-734), and its preparation and storage procedures are precisely regulated according to ISO 23317. However, despite following the regulations, SBF may precipitate during storage owing to local ion concentration fluctuations during preparation because it is a supersaturated solution of hydroxya

SUBMITTER: Dong W 

PROVIDER: S-EPMC11626438 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature