Why Bestatin Prefers Human Carnosinase 2 (CN2) to Human Carnosinase 1 (CN1).

ABSTRACT: Human carnosinases (CNs) are Xaa-His metal-ion-activated aminopeptidases that break down bioactive carnosine and other histidine-containing dipeptides. Carnosine is a bioactive peptide found in meat and prevalently used as a supplement and in functional food formulation. Nonetheless, carnosine is digested by CNs rapidly after ingestion. CNs have two isoforms (carnosinase 1 (CN1) and carnosinase 2 (CN2)), where CN1 is the main player in carnosine digestion. CNs contain a catalytic metal ion pair (Zn²⁺ for CN1 and Mn²⁺ for CN2) and two subpockets (S1 and S1' pockets) to accommodate a substrate. Bestatin (BES) has been reported to be active for CN2; however, its inhibition ability for CN1 has remained under debate, because the underlying mechanism remains unclear. This i

SUBMITTER: Toviwek B 

PROVIDER: S-EPMC11626516 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature