High-Throughput Transcriptomics Screen of ToxCast Chemicals in U-2 OS Cells.

ABSTRACT: New approach methodologies (NAMs) aim to accelerate the pace of chemical risk assessment while simultaneously reducing cost and dependency on animal studies. High Throughput Transcriptomics (HTTr) is an emerging NAM in the field of chemical hazard evaluation for establishing in vitro points-of-departure and providing mechanistic insight. In the current study, 1201 test chemicals were screened for bioactivity at eight concentrations using a 24-h exposure duration in the human- derived U-2 OS osteosarcoma cell line with HTTr. Assay reproducibility was assessed using three reference chemicals that were screened on every assay plate. The resulting transcriptomics data were analyzed by aggregating signal from genes into signature scores using gene set enrichment analysis, followed by concentrat

SUBMITTER: Bundy JL 

PROVIDER: S-EPMC11626688 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature