LncRNA 93358 Aggravates the Apoptosis of Myocardial Cells After Ischemia-Reperfusion by Mediating the PI3K/AKT/mTOR Pathway.

ABSTRACT: To investigate the impact of LncRNA 93358 on ischemia-reperfusion induced myocardial cell apoptosis and the underlying mechanism. After being subjected to hypoxia for 4 h, three models of hypoxia-reoxygenation (H/R) with reoxygenation times of 8, 16, and 24 h were established. The expression of LncRNA 93358 was detected by qPCR, and the most suitable conditions were selected for subsequent experiments. The LncRNA 93358 knockout rat myocardial cells were established by transfecting with shRNA-93358 and identified by the RT-PCR assay, followed by constructing the in vitro H/R model. H/R myocardial cells were treated with blank medium (Model), shRNA-NC (LncRNA 93358 NC), shRNA-93358 (LncRNA 93358 knock), and shRNA-93358 + LY294002 (LncRNA 93358 knockout+LY294002), respectively. Normal myocard

SUBMITTER: Cai J 

PROVIDER: S-EPMC11626694 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature